| Popis: |
The procedures, advantages, and disadvantages of the phenotyping and genotyping methods for determination of HIV-1 tropism are reviewed. The results of a genotyping determination of HIV tropism and subtypes based on sequencing of the V3 region of the gene coding for HIV envelope gp120 glycoprotein from 98 patients are presented. Lifelong use of combined antiretroviral therapy results in the need to introduce novel classes of drugs, such as CCR5 antagonists, and novel test for HIV tropism. At present, the most convenient in the clinical practice are genotyping methods based on gp120 V3-loop sequencing, which are informative and affordable. One of advantages of genotyping is the opportunity to determine HIV tropism and subtype simultaneously. Accumulating the results of forecasting non-B HIV subtypes will make it possible, with time, to enhance the accuracy of using the method in patients infected with different HIV subtypes. |
