The DSCs-Expressed CD82 Controls the Invasiveness of Trophoblast Cells via Integrinbeta1/MAPK/MAPK3/1 Signaling Pathway in Human First-Trimester Pregnancy1
| Autor: | Chuan-Ling Tang, Jun Shao, Da-Jin Li, Ming-Qing Li, Xiao-Fan Hou |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Stromal cell Decidua Trophoblast Cell Biology General Medicine Transfection Biology Cell biology Human chorionic gonadotropin Endocrinology medicine.anatomical_structure Reproductive Medicine Internal medicine embryonic structures Cancer cell medicine CD82 Autocrine signalling reproductive and urinary physiology |
| Zdroj: | Biology of Reproduction. 82:968-979 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod.109.080739 |
| Popis: | CD82 is recognized as a wide-spectrum tumor metastasis suppressor that inhibits cancer cell motility and invasiveness. At the human maternal-fetal interface, the decidua is believed to effectively limit the inappropriate invasion of trophoblasts. Here we have found the transcription and translation of CD82 in decidual stromal cells (DSCs), whereas trophoblast cells do not express CD82. The in-cell Western analysis reveals attenuation of CD82 translation in DSCs by human chorionic gonadotropin (hCG), but not by estrogen or progesterone. It is demonstrated that silencing of CD82 by RNA interference increases integrinb1, decreases TIMP1 expression in DSCs, and promotes the invasion of the first-trimester human trophoblasts in the coculture. Moreover, U0126, or anti-integrinb1 neutralizing antibody, reverses the decreased TIMP1 expression and the increased invasiveness of trophoblast cells, and the antibody also inhibits the MAPK3/1 phosphorylation induced by CD82 silence. After transfection with CD82, the invasive index of BeWo cells decreases significantly with TIMP1 increase. The results above indicate that the DSCs-expressed CD82 up-regulates the expression of TIMP1 in an autocrine manner and inhibits the invasiveness of human first-trimester trophoblast cells partly through the integrinb1/MAPK/MAPK3/1 signaling pathway. Furthermore, we have found that the mRNA and protein level of CD82 in decidua of the miscarriage is significantly higher than that of the normal early pregnancy, which implies that the abnormal higher CD82 expression in decidua restricts appropriate invasion of trophoblasts that leads to early pregnancy wastage. |
| Databáze: | OpenAIRE |
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