| Autor: |
Anna Pavlova, Songi Han, Jasmine N. Hunt, Julia H. Ortony, Ravinath Kausik, Chi Yuan Cheng, John M. Franck |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
New Journal of Physics. 13:015006 |
| ISSN: |
1367-2630 |
| Popis: |
We probe the translational dynamics of the hydration water surrounding the macromolecular surfaces of selected polyelectrolytes, lipid vesicles and intrinsically disordered proteins with site specificity in aqueous solutions. These measurements are made possible by the recent development of a new instrumental and methodological approach based on Overhauser dynamic nuclear polarization (DNP)-enhanced nuclear magnetic resonance (NMR) spectroscopy. This technique selectively amplifies 1H NMR signals of hydration water around a spin label that is attached to a molecular site of interest. The selective 1H NMR amplification within molecular length scales of a spin label is achieved by utilizing short-distance range (~r−3) magnetic dipolar interactions between the 1H spin of water and the electron spin of a nitroxide radical-based label. Key features include the fact that only minute quantities ( |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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