WIND1 Promotes Shoot Regeneration through Transcriptional Activation of ENHANCER OF SHOOT REGENERATION1 in Arabidopsis
| Autor: | Tetsuya Kurata, Kengo Morohashi, Masaru Nakata, Mariko Ohnuma, Shinichiro Komaki, Momoko Ikeuchi, Bart Rymen, Hirofumi Harashima, Erich Grotewold, Keiko Sugimoto, Masaru Ohme-Takagi, Akira Iwase |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Genetics Regulation of gene expression biology Callus formation Regeneration (biology) fungi food and beverages Cell Biology Plant Science biology.organism_classification Cell biology body regions 03 medical and health sciences 030104 developmental biology Arabidopsis Shoot Arabidopsis thaliana Enhancer Transcription factor |
| Zdroj: | The Plant Cell. 29:54-69 |
| ISSN: | 1532-298X 1040-4651 |
| DOI: | 10.1105/tpc.16.00623 |
| Popis: | Many plant species display remarkable developmental plasticity and regenerate new organs after injury. Local signals produced by wounding are thought to trigger organ regeneration but molecular mechanisms underlying this control remain largely unknown. We previously identified an AP2/ERF transcription factor WOUND INDUCED DEDIFFERENTIATION1 (WIND1) as a central regulator of wound-induced cellular reprogramming in plants. In this study, we demonstrate that WIND1 promotes callus formation and shoot regeneration by upregulating the expression of the ENHANCER OF SHOOT REGENERATION1 (ESR1) gene, which encodes another AP2/ERF transcription factor in Arabidopsis thaliana The esr1 mutants are defective in callus formation and shoot regeneration; conversely, its overexpression promotes both of these processes, indicating that ESR1 functions as a critical driver of cellular reprogramming. Our data show that WIND1 directly binds the vascular system-specific and wound-responsive cis-element-like motifs within the ESR1 promoter and activates its expression. The expression of ESR1 is strongly reduced in WIND1-SRDX dominant repressors, and ectopic overexpression of ESR1 bypasses defects in callus formation and shoot regeneration in WIND1-SRDX plants, supporting the notion that ESR1 acts downstream of WIND1. Together, our findings uncover a key molecular pathway that links wound signaling to shoot regeneration in plants. |
| Databáze: | OpenAIRE |
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