Determination of the herbicide acetochlor by fluorescence polarization immunoassay

Autor:	Yu. N. Yakovleva, M. A. Deryabina, Sergei A. Eremin, V. A. Popova
Rok vydání:	2005
Předmět:	Detection limit Chromatography medicine.diagnostic_test Chemistry Alachlor Fluorescence Binding constant Analytical Chemistry chemistry.chemical_compound Immunoassay medicine Acetochlor Metolachlor Fluorescence anisotropy
Zdroj:	Journal of Analytical Chemistry. 60:80-85
ISSN:	1608-3199 1061-9348
DOI:	10.1007/s10809-005-0063-4
Popis:	A fluorescence polarization immunoassay procedure was developed for determining the herbicide acetochlor from the group of chloroacetanilides. Conjugates of fluorescent labeled acetochlor derivatives (tracers) with glycylaminofluorescein and ethylenediaminofluorescein were synthesized. The effect of the tracer structure on the analytical characteristics of the determination and on antigen-antibody binding constants was studied. The developed immunoassay procedures are characterized by a detection limit of 10 ng/mL and an analytical range of 0.01–10 µg/mL. The procedure is highly specific; other pesticides caused no interference with the determination of acetochlor, and the percent cross-reactivities for related chloroacetanilide herbicides were no higher than 2.4% for metolachlor and lower than 0.1% for alachlor.
Databáze:	OpenAIRE
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