Cocaine Analysis in Hair: Solid-Phase Microextraction (SPME) Versus Supercritical Fluid Extraction (SFE)

Autor:	G. Iuliano, R. Marsili, O. Ghizzoni, M. Chiarotti, S. Schiavone
Rok vydání:	2007
Předmět:	Detection limit Matrix (chemical analysis) chemistry.chemical_compound Chromatography Chemistry Extraction (chemistry) Supercritical fluid extraction Benzoylecgonine Selected ion monitoring Mass spectrometry Solid-phase microextraction Pathology and Forensic Medicine
Zdroj:	Canadian Society of Forensic Science Journal. 40:143-149
ISSN:	2332-1660 0008-5030
DOI:	10.1080/00085030.2007.10757156
Popis:	The solid-phase microextraction (SPME) technique for cocaine analysis in hair was compared to supercritical fluid extraction (SFE) of the drug from the keratin matrix. After the extraction steps, the identification and quantification of cocaine were achieved by gas chromatography-mass spectrometry (GC/MS) in the selected ion monitoring (SIM) mode, using deuterated cocaine as internal standard. Owing to the mild extraction conditions, in vitro hydrolysis of cocaine to benzoylecgonine was avoided and a good correlation of quantitative data was observed comparing both of the suggested extraction procedures. The relative amount of cocaine found in hair extracted with the two mentioned methods was constantly a little bit higher when compared to classic liquid-liquid extraction procedures. A detection limit of 0.1 ng of cocaine/mg of hair sample was observed, with a linear response over a wide range of concentrations with both tested extraction techniques.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::02e86e1badf3837269b738a9ec478291 https://doi.org/10.1080/00085030.2007.10757156 Zobrazit plný text záznamu