Genome-wide gene expression of the rare, malignant Reed-Sternberg cell of Hodgkin lymphoma

Autor:	Louis M. Staudt, Kenneth C. Carter, Joseph G. Vockley, Charles E. Birse, Steven M. Ruben, Steven C. Barash, Doug Dolginow, Craig A. Rosen, Greg Lennon, Jeffrey Cossman
Rok vydání:	1999
Předmět:	Genetics Cell type Cell Cancer Biology medicine.disease Genome DNA sequencing medicine.anatomical_structure immune system diseases hemic and lymphatic diseases Gene expression medicine Gene B cell
Zdroj:	Nature Genetics. 23:40-40
ISSN:	1546-1718 1061-4036
DOI:	10.1038/14289
Popis:	We have determined the genome-wide gene expression profile of Hodgkin disease, the most common cancer of young adult Americans. Sequences were obtained from approximately 40,000 ESTs (20,000,000 bases) from Hodgkin disease sources. These included cell lines, primary tissue and living, single Reed-Sternberg cells — the exceedingly rare but biologically active malignant cell of Hodgkin lymphoma. Results of the high throughput sequencing increased the number of named, expressed genes in Hodgkin disease from 100 to 2666. We devised an algorithm to globally compare the gene expression profile of Hodgkin's disease to 100,000 ESTs from different hematopoetic cell types. The analysis supports a B cell lineage of the elusive Reed-Sternberg cell and provides a public database of the gene expression profile of Hodgkin lymphoma1 (http://www.hodgkins.georgetown.edu).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::02dd42c94a4f3ef1cb35c0f80e01d5aa https://doi.org/10.1038/14289 Zobrazit plný text záznamu Plný text ve formátu PDF