Generation of Random luxCDABE Transcriptional Fusions in the Genome of Salmonella enterica

Autor:	Concepción Panadero-Medianero, Aida Amador-Álvarez, Julia Aguilera-Herce, Barbora Benesova, Francisco Ramos-Morales, Juan J de la Rosa-Altura, Juan Luis Araujo-Garrido, Joaquín Bernal-Bayard
Rok vydání:	2020
Předmět:	Genetics Salmonella biology Salmonella enterica Operon Photorhabdus luminescens medicine Bioreporter biology.organism_classification medicine.disease_cause Gene Pathogenicity island Genome
Zdroj:	Methods in Molecular Biology ISBN: 9781071607909
Popis:	The luxCDABE operon of Photorhabdus luminescens can be used as a bioluminescent reporter to measure gene transcription nondestructively. Here we describe protocols to (1) generate random transcriptional fusions of the lux operon to genes of the Salmonella genome, (2) screen for specific fusions with constitutive expression, Salmonella pathogenicity island 1-related expression, or Salmonella pathogenicity island 2-related expression, and (3) determine the site of luxCDABE integration.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::02d8953be1358475b5ed90af20ffa5c1 https://doi.org/10.1007/978-1-0716-0791-6_13 Zobrazit plný text záznamu