Murine Colon Adenocarcinomas: Methods for Selective Culture In Vitro 2

Autor:	M. H. Tan, M. H. Goldrosen, E. D. Holyoke
Rok vydání:	1976
Předmět:	Cancer Research Pathology medicine.medical_specialty Growth medium Chemistry Trypsin Molecular biology In vitro Tissue culture chemistry.chemical_compound medicine.anatomical_structure Oncology In vivo Cell culture medicine Collagenase Fibroblast medicine.drug
Zdroj:	JNCI: Journal of the National Cancer Institute. 56:871-873
ISSN:	1460-2105 0027-8874
DOI:	10.1093/jnci/56.4.871
Popis:	Two murine colon adenocarcinoma cell lines were established from primary cultures. The MCA-38 cell line was begun by treatment of the primary culture with trypsin to remove the fibroblastoid elements. The MCA-36 epithelial cells were sensitive to trypsin; therefore, the growth medium of MCA-36 primary cultures was augmented with collagenase to release the tumor-cell elements from the fibroblast network. These tumor elements were dissociated with trypsin and placed in tissue culture. Each cell line was cultured for at least 10 passages in vitro and gave rise to tumors when reimplanted in vivo.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::02cf9bcc9d5344ca87919667780d1074 https://doi.org/10.1093/jnci/56.4.871 Zobrazit plný text záznamu