New record on karyological analysis and first study of NOR localization of parthenogenetic brahminy blind snake, Ramphotyphlops braminus (Squamata, Typhlopidae) in Thailand
Autor: | Puntivar Kaewmad, Alongklod Tanomtong, Prateep Duengkae, Yodchaiy Chuaynkern, Isara Patawang |
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Rok vydání: | 2016 |
Předmět: |
0106 biological sciences
0301 basic medicine Genetics Squamata biology Chromosome Zoology Karyotype Cell Biology Parthenogenesis Ramphotyphlops biology.organism_classification 010603 evolutionary biology 01 natural sciences Typhlopidae Nucleolar Organizer Region 03 medical and health sciences 030104 developmental biology Microchromosome Molecular Medicine Molecular Biology |
Zdroj: | The Nucleus. 59:61-66 |
ISSN: | 0976-7975 0029-568X |
DOI: | 10.1007/s13237-015-0154-z |
Popis: | This is chromosomal analysis of the brahminy blind snake, Ramphotyphlops braminus (Daudin, 1803), a newly recorded species in northeastern Thailand. Mitotic chromosomes were prepared directly from intestinal epithelia cells of five brahminy blind snakes. Conventional staining and Ag-NOR banding techniques were applied to stain the chromosomes. The results showed that the triploid chromosome number was 3n = 42, while the fundamental number (NF) was 78. The types of chromosomes were 24 macrochromosomes (19 m + 5sm) and 18 microchromosomes (9 m + 3sm + 6 t). The region adjacent to the short arm near the telomere of one in macrochromosome pair 8 showed a clearly observable nucleolar organizer region/NOR. There were no irregularly sized chromosomes related to sex. The karyotype formula for R. braminus is as follows: 3n (42) = Lm 12 + Sm 7 + Ssm 5 + 18 microchromosomes. |
Databáze: | OpenAIRE |
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