New record on karyological analysis and first study of NOR localization of parthenogenetic brahminy blind snake, Ramphotyphlops braminus (Squamata, Typhlopidae) in Thailand

Autor: Puntivar Kaewmad, Alongklod Tanomtong, Prateep Duengkae, Yodchaiy Chuaynkern, Isara Patawang
Rok vydání: 2016
Předmět:
Zdroj: The Nucleus. 59:61-66
ISSN: 0976-7975
0029-568X
DOI: 10.1007/s13237-015-0154-z
Popis: This is chromosomal analysis of the brahminy blind snake, Ramphotyphlops braminus (Daudin, 1803), a newly recorded species in northeastern Thailand. Mitotic chromosomes were prepared directly from intestinal epithelia cells of five brahminy blind snakes. Conventional staining and Ag-NOR banding techniques were applied to stain the chromosomes. The results showed that the triploid chromosome number was 3n = 42, while the fundamental number (NF) was 78. The types of chromosomes were 24 macrochromosomes (19 m + 5sm) and 18 microchromosomes (9 m + 3sm + 6 t). The region adjacent to the short arm near the telomere of one in macrochromosome pair 8 showed a clearly observable nucleolar organizer region/NOR. There were no irregularly sized chromosomes related to sex. The karyotype formula for R. braminus is as follows: 3n (42) = Lm 12 + Sm 7 + Ssm 5 + 18 microchromosomes.
Databáze: OpenAIRE