The use of matrix coating assisted by an electric field (MCAEF) to enhance mass spectrometric imaging of human prostate cancer biomarkers
| Autor: | Jun Han, Xiaodong Wang, Juncong Yang, Christoph H. Borchers, Darryl B. Hardie |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Chromatography Chemistry Cancer Sinapinic acid Mass spectrometry medicine.disease Molecular biology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine Tumor Protein D52 030220 oncology & carcinogenesis Heat shock protein medicine Cancer biomarkers Protein kinase A Glycoprotein Spectroscopy |
| Zdroj: | Journal of Mass Spectrometry. 51:86-95 |
| ISSN: | 1076-5174 |
| Popis: | In this work, we combined a newly developed matrix coating technique - matrix coating assisted by an electric field (MCAEF) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) to enhance the imaging of peptides and proteins in tissue specimens of human prostate cancer. MCAEF increased the signal-to-noise ratios of the detected proteins by a factor of 2 to 5, and 232 signals were detected within the m/z 3500-37500 mass range on a time-of-flight mass spectrometer and with the sinapinic acid MALDI matrix. Among these species, three proteins (S100-A9, S100-A10, and S100-A12) were only observed in the cancerous cell region and 14 proteins, including a fragment of mitogen-activated protein kinase/extracellular signal-regulated kinase kinase kinase 2, a fragment of cAMP-regulated phosphoprotein 19, 3 apolipoproteins (C-I, A-I, and A-II), 2 S100 proteins (A6 and A8), β-microseminoprotein, tumor protein D52, α-1-acid glycoprotein 1, heat shock protein β-1, prostate-specific antigen, and 2 unidentified large peptides at m/z 5002.2 and 6704.2, showed significantly differential distributions at the p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text