| Autor: |
Hlromasa Ishii, Kanji Wakabayashi, Hidetsugu Saito, Tamako Takagi, Nobuhiro Nakamoto, Satoshi Kurita, Shinichiro Tada |
| Rok vydání: |
2001 |
| Předmět: |
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| Zdroj: |
Trends in Gastroenterology and Hepatology ISBN: 9784431679936 |
| DOI: |
10.1007/978-4-431-67895-3_26 |
| Popis: |
Interferon regulatory factor-1 is an important transcription factor in the interferon system. We have demonstrated that the growth inhibitory effect of type 1 interferons on human hepatoma cells correlates with levels of transcription of the interferon regulatory factor-1 gene. In this study we investigated whether mutations in this gene cause different responses to type 1 interferons. DNA from several human hepatoma cell lines was investigated, and nucleotide sequences of the interferon regulatory factor-1 gene were determined. Several point mutations were detected in intron 8 of the gene, and one was found to cause deletion of BamHI cleavage sites. We found several point mutations in the upstream region of the interferon regulatory factor-1 exon 1, and the luciferase promoter assay demonstrated that these mutations modified its promoter activity. These results suggest that polymorphisms of the interferon regulatory factor-1 gene partially contribute to determining the response of cancer cells to growth inhibitory effect of type 1 interferons. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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