1H-NMR analysis of microbial-derived organic acids in primary root carious lesions and saliva

Autor: Martin Grootveld, Sean Seddon, Christopher J.L. Silwood, Andrew W.D. Claxson, Angela Sheerin, Edward Lynch
Rok vydání: 1999
Předmět:
Zdroj: NMR in Biomedicine. 12:345-356
ISSN: 1099-1492
0952-3480
DOI: 10.1002/(sici)1099-1492(199910)12:6<345::aid-nbm580>3.0.co;2-c
Popis: In addition to lowered pH values, the molecular profile and concentrations of microbial-derived organicacids in carious dentin are important demineralization parameters involved in the induction, development and progression of dental caries. High-resolution proton ( 1H) NMR spectroscopy was employed to examine the organic acid status of primary root carious lesions. 1H-NMR analysis of post-neutralized perchloric acid extracts of active carious lesions revealed that at an operating frequency of 600 MHz, the 1H-NMR-detectable organic acid composition of carious dentin samples (mean molecular percentage content ± standard error; the mean molecular percentage content is defined here as the mean of the concentration of each 1H-NMR-visible organic acid/anion expressed as a percentage of total 1H-NMR-detectable organic acid/anion level in each sample) was acetate 51 ± 2%, formate 37 ± 2%, lactate 5 ± 1%, propionate 3 ± 0.8%, pyruvate 2.4 ± 0.3%, n-butyrate 1.2 ± 0.2%; succinate 0.1 ± 0.1%; iso-butyrate, n- and iso-valerate, and n- and iso-caproate (total)
Databáze: OpenAIRE
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