Identification and determination of aminopeptidase activities secreted by lemon balm

Autor:	V. Blanarikova, M. Korenova, J. Stano, Karol Mičieta
Rok vydání:	2007
Předmět:	chemistry.chemical_classification Chromatography biology Plant Science General Chemistry Aminopeptidase General Biochemistry Genetics and Molecular Biology Enzyme assay Amino acid chemistry Biochemistry biology.protein Extracellular Lemon balm Intracellular
Zdroj:	Chemistry of Natural Compounds. 43:201-204
ISSN:	1573-8388 0009-3130
Popis:	Using synthetic substrates, an uncomplicated and sensitive procedure for the identification and determination of extracellular aminopeptidase was developed. The β-naphthylamides of the amino acids were applied for the identification of extracellular aminopeptidase, whereas the 4-(phenylazo) phenylamides of the amino acids were used for the determination of intra-and extracellular aminopeptidase activity. The results show a 81.8–88.9% intracellular and 11.1–18.2% extracellular distribution of the studied enzyme activity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::02a957110029bed12f3a4cff53a21deb https://doi.org/10.1007/s10600-007-0078-1 Zobrazit plný text záznamu Plný text ve formátu PDF