Detection and Molecular Characterization of ‘Candidatus Liberibacter asiaticus’ and Citrus Tristeza Virus Associated with Citrus Decline in Bhutan
| Autor: | Phuntsho Wangdi, Sunil B. Kokane, Dilip Kumar Ghosh, Amol D. Kokane, Siddarame Gowda, Mrugendra G. Gubyad, A. A. Murkute, Jigme Tenzin, Ashwani Sharma |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Pathogen detection biology Candidatus Liberibacter asiaticus food and beverages Citrus tristeza virus Plant Science biology.organism_classification 16S ribosomal RNA 01 natural sciences 03 medical and health sciences Horticulture 030104 developmental biology Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Phytopathology®. 111:870-881 |
| ISSN: | 1943-7684 0031-949X |
| Popis: | Citrus, mainly mandarin (Citrus reticulata Blanco), is an economically important fruit crop in Bhutan. Despite having favorable agroclimatic conditions for citrus cultivation, the early decline of fruit-bearing orchards coupled with low crop productivity is a major concern among citrus growers. During a recent survey, an association of ‘Candidatus Liberibacter asiaticus’ (citrus greening) and citrus tristeza virus (CTV), either singly or as mixed infections in declined citrus trees, was recorded in all four major citrus-growing districts (Tsirang, Dagana, Zhemgang, and Sarpang). Using PCR-based diagnosis, a higher incidence of citrus greening (27.45%) and tristeza (70.58%) was observed in symptomatic field samples. Detection and characterization of ‘Ca. L. asiaticus’ was performed based on the 16S ribosomal DNA, prophage gene, 50S ribosomal rplA-rplJ gene, and tandem repeats of the CLIBASIA_01645 locus. Similarly, the coat protein, p23, and p18 genes were used as genetic markers for the detection and characterization of Bhutanese CTV. The ‘Ca. L. asiaticus’ isolates from Bhutan segregated into classes II and III based on the CLIBASIA_01645 locus, analogous to Indian isolates from the northeast region and Term-A based on the CLIBASIA_05610 locus. CTV isolates of Bhutan were observed as closely related to the VT strain, which is considered to be the most devastating. To the best of our knowledge, this is the first study on molecular characterization of ‘Ca. L. asiaticus’ and CTV isolates and their association with citrus decline in Bhutan. |
| Databáze: | OpenAIRE |
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