Validation of a HPLC-ECD method for the detection of adrenaline-GSH adducts in biological samples

Autor:	Renata Silva, Félix Carvalho, Maria de Lourdes Bastos, Márcia Carvalho, Fernando Remião, Helena Carmo, Sílvia Boldt, Vera Marisa Costa
Rok vydání:	2006
Předmět:	chemistry.chemical_compound Chromatography Chemistry General Medicine Perchloric acid Glutathione Toxicology Adduct Hplc ecd
Zdroj:	Toxicology Letters. 164:S132
ISSN:	0378-4274
DOI:	10.1016/j.toxlet.2006.06.277
Popis:	Adrenaline:(25 nM to 3.75 mM)GSH: 3.75 mMTyrosinase: 0.4 units/mLPhosphate Buffer pH 7.4Vortex 2 minroom temperatureRest 10 minroom temperatureTransfer 100 µL to 400 µL Human PlasmaInject supernatantinto HPLCExctractionprocedureORStop Reaction with250µL Perchloric acid 15%Centrifuge13000 rpm/5 min4oC
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::029ebf2c1ba80f5f6e7e824d29648d36 https://doi.org/10.1016/j.toxlet.2006.06.277 Zobrazit plný text záznamu Full Text from ScienceDirect