Bacterial Biosynthetic P450 Enzyme PikCD50N: A Potential Biocatalyst for the Preparation of Human Drug Metabolites

Autor:	Xiaohui Liu, Shengying Li, Xingwang Zhang, Jiawei Guo, Wei Zhang, Gang Zhang, Li Ma, Lei Du, Fengwei Li, Fangyuan Cheng, Pradeepraj Durairaj, Dandan Tang, Xiangtian Long
Rok vydání:	2021
Předmět:	Streptomyces venezuelae chemistry.chemical_classification biology Organic Chemistry Mutant Substrate (chemistry) biology.organism_classification chemistry.chemical_compound Enzyme Biochemistry Biosynthesis chemistry Pikromycin Drug metabolism Bacteria
Zdroj:	The Journal of Organic Chemistry. 86:14563-14571
ISSN:	1520-6904 0022-3263
DOI:	10.1021/acs.joc.1c01407
Popis:	Human drug metabolites (HDMs) are important chemicals widely used in drug-related studies. However, acquiring these enzyme-derived and regio-/stereo-selectively modified compounds through chemical approaches is complicated. PikC is a biosynthetic P450 enzyme involved in pikromycin biosynthesis from the bacterium Streptomyces venezuelae. Here, we identify the mutant PikCD50N as a potential biocatalyst, with a broad substrate scope, diversified product profile, and high catalytic efficiency, for preparation of HDMs. Remarkably, PikCD50N can mediate the drug-metabolizing reactions using the low-cost H2O2 as a direct electron and oxygen donor.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::02972bf0ef268aaf38843e93afcbb3af https://doi.org/10.1021/acs.joc.1c01407 Zobrazit plný text záznamu