Impact of Major Orthopedic Surgery On Regulatory CD4+ T Lymphocytes and Cytokines in Transfused and Non-Transfused Patients
| Autor: | Akira Ishida, José Orlando Bordin, Edmilson Takehiro Takata, Otavio C. G. Baiocchi, Akemi Kuroda Chiba, Joyce M. K. Silva, Priscilla Brito Silva, Luciana de Andrade Pereira |
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| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
Blood transfusion business.industry medicine.medical_treatment Lymphocyte Immunology FOXP3 Cell Biology Hematology Biochemistry Gastroenterology Interleukin 10 medicine.anatomical_structure Internal medicine medicine Cytotoxic T cell Cytokine secretion Interleukin 17 IL-2 receptor business |
| Zdroj: | Blood. 120:2286-2286 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v120.21.2286.2286 |
| Popis: | 2286 It is known that major surgery and allogeneic blood transfusion mediate immunosuppression by interfering on cytokine secretion, lymphocyte count and cytotoxic response. However, which cytokines and lymphocyte subpopulations participate in such immunosuppressive state remains poorly understood. Regulatory T cells (Tregs) are suppressive CD4(+) cells with a central role in immunosuppression of trauma victims, cancer patients, and transplant recipients. Several markers have been identified with regulatory properties, such as FOXP3, CTLA-4, GITR and the recently described CD69. Recently, allogeneic blood transfusion has been shown capable of inducing regulatory CD4+FOXP3+ T cells in vitro. Purpose: In this study, we aimed to investigate regulatory CD4+ T cells induction and cytokine profile in transfused and non-transfused surgical patients. Patients and Methods: Thirty-five patients undergoing elective hip replacement were recruited for this study and prospectively evaluated. Blood samples were obtained before surgery (D0) and on days 1 (D1) and 4 (D4) after surgery. Quantification of regulatory T lymphocytes was done by flow cytometry using CD4, CD25, FoxP3, CTLA-4, GITR and CD69, while cytokines (IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-17A, TNF-α, IFN-γ and TGF-β) levels were determined by multiplexed immunoassay system. Results: From the 35 patients recruited for this study, 18 (51.4%) received allogeneic non-leukodepleted red cells transfusion during or after surgery. There were no significant differences in clinical or epidemiological characteristics between transfused and non-transfused groups, except for the duration of surgery, being longer in transfused patients (p |
| Databáze: | OpenAIRE |
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