Control of the Selectivity of Separation and the Determination of Anthocyanins of Fruits of Vaccinium Family Plants Using Acetonitrile–Formic Acid–Water Eluents

Autor: L. A. Deineka, Ya. Yu. Kul’chenko, I. P. Blinova, E. Yu. Oleinits, V. I. Deineka
Rok vydání: 2020
Předmět:
Zdroj: Journal of Analytical Chemistry. 75:1443-1450
ISSN: 1608-3199
1061-9348
DOI: 10.1134/s1061934820090087
Popis: It is shown that the selectivity of the separation of 15 anthocyanins from an extract of bilberries (3-galactosides, 3-glucosides and 3-arabinosides of the five main anthocyanins: cyanidin, peonidin, delphinidin, petunidin, and malvidin) with eluents containing acetonitrile and 10 vol % HCOOH in water can be controlled by varying the concentration of acetonitrile and separation temperature. A gradient mode at a separation temperature of 25°C is proposed for the rapid separation of all components without the introduction of methanol into the mobile phase. The developed method is used to determine anthocyanins in a number of commercial fruit products of plants of the Vaccinium family: bilberries, bog bilberries, cranberries, and lingonberries. A chromatographic version for eliminating the absorbance of polymer anthocyanins in the determination of monomeric compounds in complex mixtures is proposed as an alternative to differential spectrophotometry.
Databáze: OpenAIRE
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