Liver-specific metabolomics characterizes the hepatotoxicity of Dioscorea bulbifera rhizome in rats by integration of GC-MS and 1H-NMR
| Autor: | Hui-Jun Li, Zi-Tian Wu, Zhuo-Qing Li, Dong-Sheng Zhao, Ling-Li Wang, Li-Long Jiang, Wei Shi, Ping Li |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Pharmacology chemistry.chemical_classification Purine Liver injury Bile acid biology medicine.drug_class Dioscorea bulbifera biology.organism_classification medicine.disease Amino acid Rhizome 03 medical and health sciences chemistry.chemical_compound Metabolic pathway 030104 developmental biology Metabolomics chemistry Drug Discovery medicine |
| Zdroj: | Journal of Ethnopharmacology. 226:111-119 |
| ISSN: | 0378-8741 |
| DOI: | 10.1016/j.jep.2018.08.014 |
| Popis: | Ethnopharmacological relevance Dioscorea bulbifera rhizome (DBR), one type of herbal medicine, is extensively used in both Indian and Chinese system of traditional medicine. It has been effective in treating various diseases, such as sore throat, struma, and tumors. However, more and more clinical investigations have suggested that DBR can cause liver injury. Aim of the study In the present study, we aimed to characterize the corresponding molecular changes of liver dysfunction and reveal overall metabolic and physiological mechanisms of the subchronic toxic effect of DBR. Materials and methods A liver-specific metabolomics approach integrating GC-MS and 1H-NMR was developed to assess the hepatotoxicity in rats after DBR exposure for 12 weeks. Multivariate statistical analysis and pattern recognition were employed to examine different metabolic profiles of liver in DBR-challenged rats. Results A total of 61 metabolites were screened as significantly altered metabolites, which were distributed in 43 metabolic pathways. The correlation network analysis indicated that the hub metabolites of hepatotoxicity could be mainly linked to amino acid, lipid, purine, pyrimidine, bile acid, gut microflora, and energy metabolisms. Notably, purine, pyrimidine, and gut microflora metabolisms might be novel pathways participating in metabolic abnormalities in rats with DBR-triggered hepatic damage. Conclusions Our results primarily showed that the liver-specific metabolic information provided by the different analytical platforms was essential for identifying more biomarkers and metabolic pathways, and our findings provided novel insights into understand the mechanistic complexity of herb-induced liver injury. |
| Databáze: | OpenAIRE |
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