Suppression of c-myc is a critical step in glucocorticoid-induced human leukemic cell lysis

Autor:	E B Thompson, D. V. Harbour, Ramachandran Thulasi
Rok vydání:	1993
Předmět:	Lysis Cell growth Cell Biology Transfection Biology Biochemistry Cell biology Cytolysis Glucocorticoid receptor Mammary tumor virus Cancer research medicine Cytotoxicity Molecular Biology Glucocorticoid medicine.drug
Zdroj:	Journal of Biological Chemistry. 268:18306-18312
ISSN:	0021-9258
DOI:	10.1016/s0021-9258(17)46845-1
Popis:	Glucocorticoids evoke cytolysis in clonal human leukemic CEM-C7 cells. Suppression of c-myc mRNA by dexamethasone closely correlates with cell lysis only in CEM clones with both glucocorticoid receptor and intact lysis functions. We tested the theory that c-myc repression is essential for glucocorticoid-induced lymphocytolysis by preventing down-regulation of c-myc gene in the presence of dexamethasone and by reducing c-myc mRNA levels with antisense oligonucleotides. We find that sustained expression of c-myc provides resistance to dexamethasone-induced lysis, and antisense c-myc oligomers induce cell lysis. The lethal effects of dexamethasone in these leukemic cells appear to involve reduction of c-myc below the levels required to maintain cellular growth and integrity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::027a6427f08c687d12d43d3aa935ab97 https://doi.org/10.1016/s0021-9258(17)46845-1 Zobrazit plný text záznamu