The role of ABCG2 in maintaining the viability and proliferative activity of bone marrow mesenchymal stem cells in hypoxia
| Autor: | A. G. Poleshko, I. D. Volotovski |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Messenger RNA Reactive oxygen species Abcg2 biology Basic fibroblast growth factor Mesenchymal stem cell Biophysics Hypoxia (medical) Molecular biology In vitro Cell biology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine chemistry 030220 oncology & carcinogenesis medicine biology.protein Doubling time medicine.symptom |
| Zdroj: | Biophysics. 61:271-276 |
| ISSN: | 1555-6654 0006-3509 |
| Popis: | Hypoxia (5% O2) and the basic fibroblast growth factor (bFGF) were shown to reduce the doubling time of bone marrow mesenchymal stem cells (MSCs) in vitro, indicating an increase in cell proliferation. In addition, abcg2 expression at both the mRNA and protein levels increased in MSCs that were exposed to hypoxia and bFGF. The two factors acting together potentiated the effects of hypoxia in MSCs. Blocking the functional activity of ABCG2 led to a higher production of reactive oxygen species (ROS) in MSCs. Hypoxia and bFGF were tested for effects on the MSC protein profile. These findings, combined with the published data, implicate ABCG2 expression and function in maintaining the viability and proliferative activity of MSCs that are cultured in hypoxia, with ABCG2 playing a protective role. |
| Databáze: | OpenAIRE |
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