Synthesis, structural and spectral characterization, and in vitro nuclease activity of new thiosemicarbazone derivatives
| Autor: | Teoh Siang Guan, Mouayed A. Hussein |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Gel electrophoresis
Nuclease biology 010405 organic chemistry Stereochemistry Substituent Crystal structure Carbon-13 NMR 010402 general chemistry 01 natural sciences 0104 chemical sciences Crystallography chemistry.chemical_compound chemistry Agarose gel electrophoresis biology.protein Methylene Semicarbazone |
| Zdroj: | European Journal of Chemistry. 7:1-7 |
| ISSN: | 2153-2257 2153-2249 |
| DOI: | 10.5155/eurjchem.7.1.1-7.1354 |
| Popis: | Two new compounds, ( E )-2-(3-ethoxy-2-hydroxybenzylidene)hydrazinecarbothioamide (1) and ( E )- N -ethyl-2-((2-hydroxynaphthalen-1-yl)methylene)hydrazinecarbothioamide (2) have been synthesized. The prepared compounds have been characterized by CHN analysis, FT-IR, UV-Vis, 1 H and 13 C NMR spectroscopic techniques as well as the fluorescence emission spectroscopy. The molecular structures of the compounds have also been determined by X-ray single crystal diffraction analysis. The crystal structures revealed that the compounds are remain as a thione form in the solid state and are different in their geometrical, conformational and symmetrical structures. The nuclease activity of compounds to cleave pBR 322 has been investigated using agarose gel electrophoresis assay. The compound 2 revealed a significant nuclease activity which was attributed to the lipophilic properties that provided by substituent moieties of the compound. |
| Databáze: | OpenAIRE |
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