QUANTITATIVE DETERMINATION AND PREPARATIVE ISOLATION OF TWO MAJOR ALKALOIDS FROM THE VIETNAMESE MEDICINAL HERB EVODIAE FRUCTUS
| Autor: | T. Kien Nguyen, X. Dao T. Nguyen, Kyeong Ho Kim, K. Ngan H. Nguyen, M. Phuong Nguyen, N. Van T. Nguyen |
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| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | International Journal of Pharmacy and Pharmaceutical Sciences. :26-32 |
| ISSN: | 0975-1491 2656-0097 |
| DOI: | 10.22159/ijpps.2019v11i11.34887 |
| Popis: | Objective: To develop a simple and accurate HPLC-DAD method for simultaneous determination, the content of major components: limonin, evodiamine, and rutaecarpine in Evodiae fructus and evaluation the quality of Evodiae fructus sold in markets. Methods: Open column chromatography was used to separate and purify rutaecarpine and evodiamine, the two major alkaloids from Evodiae fructus extract as a laboratory standard. Chromatographic separation was achieved using a Germini C18 column (150 mm × 4.6 mm I.D., 5 µm), detected at 210 nm. The mobile phase consisted of acetonitrile (A), methanol (B), and water (C). The validated method simultaneously determined alkaloid content in 40 batches of samples collected from markets in different regions of Vietnam. Results: In one-step purification, our method yielded 326 mg of rutaecarpine and 128 mg of evodiamine from 3.2 g of crude extract, with purities of 98.9 and 98.5%, respectively. The structures of these compounds were identified using 1H NMR and 13C NMR. There was a significant correlation between alkaloid content and fruit size, with a Spearman correlation coefficient of>0.5 (p |
| Databáze: | OpenAIRE |
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