Sodium Valproate Sensitizes Non-Small Lung Cancer A549 Cells to γδ T-Cell-Mediated Killing through Upregulating the Expression of MICA
| Autor: | Qiong-ya Li, Fa-wang Du, Zheng-guang He, Xian-zhi Du, Juan Wang |
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| Rok vydání: | 2013 |
| Předmět: |
A549 cell
biology Chemistry medicine.drug_class Health Toxicology and Mutagenesis T cell medicine.medical_treatment Histone deacetylase inhibitor General Medicine Immunotherapy Toxicology Major histocompatibility complex Biochemistry medicine.anatomical_structure Cancer immunotherapy Apoptosis Immunology medicine biology.protein Cancer research Molecular Medicine lipids (amino acids peptides and proteins) Cytotoxicity Molecular Biology |
| Zdroj: | Journal of Biochemical and Molecular Toxicology. 27:492-498 |
| ISSN: | 1095-6670 |
| Popis: | Major histocompatibility complex (MHC) class I chain-related protein A (MICA) is involved in γδ T-cell recognition of target tumor cells. The aim of this study was to investigate the feasibility of utilization of sodium valproate (VPA), a histone deacetylase inhibitor, to sensitize non-small cell lung cancer A549 cells to γδ T-cell-mediated killing. VPA induced a dose-dependent increase in the mRNA and protein expression of MICA in A549 cells. γδ T cells showed cytotoxicity to A549 cells, which was increased by about 50% in the presence of VPA. The concomitant addition of MICA antibody significantly attenuated the VPA-mediated sensitization to γδ T-cell killing. VPA enhanced the cleavage of caspase-3 and caspase-9 in A549 cells cocultured with γδ T cells, and such enhancement was reversed by the MICA antibody. In conclusion, VPA sensitizes tumor cells to γδ T-cell-mediated cytotoxicity through the upregulation of MICA and may thus have benefits in improving γδ T-cell-based cancer immunotherapy. © 2013 Wiley Periodicals, Inc. J BiochemMol Toxicol 27:492-498, 2013; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21513 |
| Databáze: | OpenAIRE |
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