Sfmbt Co-purifies with Hangover and SWI/SNF-Remodelers in Drosophila melanogaster

Autor:	M. M. Erokhin, Pavel Georgiev, D. V. Lomaev, Yu. V. Shidlovskii, D. A. Chetverina
Rok vydání:	2021
Předmět:	biology Biophysics macromolecular substances General Chemistry General Medicine biology.organism_classification Biochemistry Interactome SWI/SNF Chromatin Cell biology Peptide sequencing Drosophila melanogaster Psychological repression
Zdroj:	Doklady Biochemistry and Biophysics. 500:304-307
ISSN:	1608-3091 1607-6729
DOI:	10.1134/s1607672921050069
Popis:	Polycomb group (PcG) proteins are chromatin-associated factors involved in the repression of gene transcription. In the present study, we characterized the interactome of the Sfmbt factor at the embryonic stage of development. For this, the Sfmbt protein complex was affinity purified from the nuclear extract, followed by highly specific peptide sequencing (IP/LC-MS). As a result, a number of previously uncharacterized Sfmbt interactions were discovered. In particular, Sfmbt top-interacting proteins include the DNA-binding protein Hangover and components of the SWI/SNF family of chromatin remodelers.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::023c6a6e5af15fdc938da45903277f4d https://doi.org/10.1134/s1607672921050069 Zobrazit plný text záznamu Plný text ve formátu PDF