Enrichment as a screening method for a high-growth-rate microalgal strain under continuous cultivation system
| Autor: | Min Gyu Sung, Jong-Hee Kwon, Won-Sub Shin, Hansol Lee, Simon MoonGeun Jung, Kwontack Hwang |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
biology 020209 energy Chlorella vulgaris Biomedical Engineering Chlamydomonas reinhardtii Turbidostat Photobioreactor Biomass Bioengineering 02 engineering and technology biology.organism_classification 01 natural sciences Applied Microbiology and Biotechnology Biofuel 010608 biotechnology Biodiesel production Botany 0202 electrical engineering electronic engineering information engineering Food science Industrial and production engineering Biotechnology |
| Zdroj: | Biotechnology and Bioprocess Engineering. 21:268-273 |
| ISSN: | 1976-3816 1226-8372 |
| Popis: | Microalgae are a promising feedstock for renewable biodiesel production. High productivity of biodiesel production from microalgae is directly related to growth rate as well as lipid content of cells. In the present study, an enrichment process in a continuous cultivation system was developed to screen a high-growth-rate microalga from a mixed culture of microalgal species; Chlorella vulgaris, Chlorella protothecoides, and Chlamydomonas reinhardtii were used as test organisms for our experiments. The time-dependent washout of mixed microalgal pool was executed to successfully enrich the C. reinhardtii, which exhibits the higher growth rate than C. vulgaris and C. protothecoides under turbidostat conditions within 75 h. The domination of C. reinhardtii in the mixed culture was validated by on-line monitoring of growth rate and flowcytometric analysis. For the time-efficient production of microalgal biomass, this screening process has a high potential to segregate the fast-growing microalgal strains from the pool of various uncharacterized microalgal species and random mutants. |
| Databáze: | OpenAIRE |
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