| Autor: |
Sheldon M. Schuster, N. E. Pfeiffer, Dwane E. Wylie |
| Rok vydání: |
1987 |
| Předmět: |
|
| Zdroj: |
Journal of Immunological Methods. 97:1-9 |
| ISSN: |
0022-1759 |
| DOI: |
10.1016/0022-1759(87)90098-6 |
| Popis: |
Differences in antigen-binding capacity of a monoclonal antibody coupled to Sepharose under varying conditions were explored. The extent of cyanogen bromide activation, and the pH of the coupling reaction had a profound effect upon the rate of antibody coupling, but only small differences in antigen-binding capacity were observed if the antibody coupling reaction was terminated when 80–90% of the antibody was covalently coupled to Sepharose. However, if antibody was incubated with activated resin until 100% coupling was attained, the antigen-binding capacity of the resulting immunoadsorbent decreased significantly. Monoclonal antibody coupled to Sepharose via an N-hydroxysuccinimide ester linkage and approximately half the antigen-binding capacity of antibody coupled by CNBr activation. Concentrations of monoclonal antibodies as high as 13 mg/ml of packed resin could be used without noticeable steric hindrance. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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