Desiccation Tolerance of Spermatozoa Dried at Ambient Temperature: Production of Fetal Mice1
| Autor: | Bharat D. Nath, Liben Zhu, John D. Biggers, Mehmet Toner, Lynda K. McGinnis, Sankha Bhowmick, Joel A. Lawitts |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
urogenital system
medicine.medical_treatment Embryo Cell Biology General Medicine Biology Insemination Sperm Intracytoplasmic sperm injection Desiccation tolerance Andrology Freeze-drying medicine.anatomical_structure Reproductive Medicine Botany medicine Blastocyst Desiccation reproductive and urinary physiology |
| Zdroj: | Biology of Reproduction. 68:1779-1786 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod.102.009407 |
| Popis: | Long-term preservation of mouse sperm by desiccation is economically and logistically attractive. The current investigation is a feasibility study of the preservation of mouse sperm by convective drying in an inert gas (nitrogen). Mouse sperm from the B6D2F1 strain isolated in an EGTA-supplemented Tris-HCl buffer were dried using three different drying rates and were stored for 18-24 h at 4 degrees C. The mean final moisture content was |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|