Molecular responses of arbuscular mycorrhizal fungi in tolerating root rot of trifoliate orange
| Autor: | Tian Li, Kamil Kuca, Cheng Shen, Wu QiangSheng, Zou YingNing, Popy Bora |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
biology Inoculation fungi Defence mechanisms food and beverages RNA-Seq 04 agricultural and veterinary sciences Plant Science Horticulture Plant disease resistance biology.organism_classification 01 natural sciences Microbiology Trifoliate orange Transcriptome 040103 agronomy & agriculture Root rot 0401 agriculture forestry and fisheries Agronomy and Crop Science Gene 010606 plant biology & botany |
| Zdroj: | Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 48:558-571 |
| ISSN: | 1842-4309 0255-965X |
| DOI: | 10.15835/nbha48211916 |
| Popis: | Arbuscular mycorrhizal fungi (AMF) enhance plant disease resistance, while the underlying mechanisms in the molecular levels are not yet known. In this study, five-leaf-old trifoliate orange seedlings were inoculated with Funneliformis mosseae for 14 weeks and subsequently were infected by a citrus root rot pathogen Phytophthora parasitica by 7 days. The transcriptome results by Illumina HiSeq 4000 revealed that the percentage of Q30 bases reached 92.99% or above, and 29696 unigenes were annotated in a total of 63531 unigenes. 654 and 103 differentially expressed genes (DEGs) were respectively annotated in AMF-inoculated versus non-AMF-inoculated plants under non-infection and infection with P. parasitica, respectively, whilst these DEGs were related to defense mechanisms, signal transduction mechanisms and secondary metabolites biosynthesis. Forty-two genes were functionally annotated as the putative 'defense mechanism', whilst AMF inoculation induced 1 gene down-regulated and 3 genes up-regulated under P. parasitica infection. AMF inoculation stimulated more genes linked to signal transduction mechanism down-regulated than non-AMF plants. Eight genes were involved in secondary metabolites biosynthesis in AMF versus non-AMF seedlings under P. parasitica-infection conditions. Such transcriptome database provided total information in the molecular levels regarding mycorrhizal roles in tolerating Phytophthora parasitica infection. |
| Databáze: | OpenAIRE |
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