Sugarcane Syrup Improves Xylitol Bioproduction from Sugarcane Bagasse and Straw Hemicellulosic Hydrolysate
| Autor: | Maria das Graças de Almeida Felipe, Andrés Felipe Hernández-Pérez, Júlio César dos Santos, Priscila Vaz de Arruda, Alejandra Camila Chaves-Villamil |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Environmental Engineering Sucrose Renewable Energy Sustainability and the Environment Chemistry 020209 energy 02 engineering and technology Xylose Straw Xylitol 01 natural sciences Hydrolysate chemistry.chemical_compound 010608 biotechnology 0202 electrical engineering electronic engineering information engineering Fermentation Food science Bioprocess Bagasse Waste Management and Disposal |
| Zdroj: | Waste and Biomass Valorization. 11:4215-4224 |
| ISSN: | 1877-265X 1877-2641 |
| DOI: | 10.1007/s12649-019-00742-6 |
| Popis: | To evaluate the supplementation of the sugarcane bagasse and straw hemicellulosic hydrolysate with sugarcane syrup, which is a sucrose-rich co-product, for xylitol production by Candida guilliermondii FTI 20037. Recently, we demonstrated that sucrose supplementation to sugarcane straw hemicellulosic hydrolysate favoured this bioprocess. A mixture 1:1 of sugarcane bagasse and straw was submitted to dilute-acid hydrolysis with H2SO4 to obtain the hemicellulosic hydrolysate, which was concentrated, detoxified and autoclaved. Three sugarcane syrup concentrations (0.83, 2.50 and 3.33% v/v) were added to the hemicellulosic hydrolysate, which was also supplemented with rice bran extract, (NH4)2SO4, CaCl2·2H2O. Next, a full factorial design was performed to assess the replacement of conventional nutrients by sugarcane syrup. Fermentations were performed in 125 mL Erlenmeyer flasks containing 50 mL of fermentation medium, for 48 h at 30 °C and 200 rpm. Improvements of 16.85% and 12.24% in xylose uptake rate (1.04 ± 0.01 g L−1 h−1) and xylitol volumetric productivity (0.55 ± 0.02 g L−1 h−1), respectively, were achieved with syrup supplementation (2.50% v/v), which led to a final xylitol titer of 26.19 ± 0.02 g L−1. Conventionally employed nutrients (rice bran extract, (NH4)2SO4 and CaCl2·2H2O) did not have significant influence on xylitol production when sugarcane syrup was added to the fermentation medium. Sugarcane syrup as a nutritional supplement improved xylitol production from a mixture of bagasse and straw, becoming an alternative to reduce costs in the formulation of the fermentation medium and contributing to the integration of this bioprocess in a sugarcane biorefinery. |
| Databáze: | OpenAIRE |
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