Polyphyllin VI Induces Apoptosis and Autophagy via Reactive Oxygen Species Mediated JNK and P38 Activation in Glioma
| Autor: | Libo Hu, Junhua Wang, Wei Liu, Hong-Yu Yuan, Yongmei Song, Yuqi Zhang, Zi-Tong Zhao, Yi Chai, Xin Pan |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Reactive oxygen species medicine.diagnostic_test Cell growth p38 mitogen-activated protein kinases Autophagy medicine.disease Flow cytometry 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Oncology chemistry Cell culture Apoptosis 030220 oncology & carcinogenesis Glioma Cancer research medicine Pharmacology (medical) |
| Zdroj: | OncoTargets and Therapy. 13:2275-2288 |
| ISSN: | 1178-6930 |
| Popis: | Background Polyphyllin VI (PPVI), a bioactive component derived from a traditional Chinese herb Paris polyphylla, exhibits potential antitumor activity against hepatocellular carcinoma, as well as breast and lung cancers. However, its effect on glioma remains unknown. Methods Five glioma cell lines (U251, U343, LN229, U87 and HEB) and an animal model were employed in the study. Anti-proliferation effects of PPVI were first determined using CCK-8 cell proliferation and clone formation assays, then reactive oxygen species (ROS), cell cycle progression and apoptosis effects measured by flow cytometry. The effect of PPVI on protein expression was quantified by Western blot analysis. Results Data showed that PPVI inhibited the proliferation of glioma cell lines by modulating the G2/M phase. Additionally, incubation of cells with PPVI promoted apoptosis, autophagy, increased accumulation of ROS and activated ROS-modulated JNK and p38 pathways. On the other hand, N-acetyl cysteine, a ROS inhibitor, attenuated PPVI-triggered effects. Furthermore, JNK and p38 inhibitors ameliorated PPVI-triggered autophagy and apoptosis in glioma cells. In vivo assays showed that PPVI inhibited tumor growth of U87 cell line in nude mice. Conclusion Overall, these data suggested that PPVI might be an effective therapeutic agent for glioma. |
| Databáze: | OpenAIRE |
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