Is the Medium Still the Message? Culture-Independent Diagnosis of Gastrointestinal Infections
| Autor: | Holly S. Greenwald, Neil Sood, Frank K. Friedenberg, Gary Carbell |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Microbiological culture Physiology business.industry Gastroenterology Hepatology medicine.disease Inflammatory bowel disease Gastrointestinal infections law.invention Diarrhea law Current practice Internal medicine medicine medicine.symptom Intensive care medicine business Culture independent Polymerase chain reaction |
| Zdroj: | Digestive Diseases and Sciences. 67:16-25 |
| ISSN: | 1573-2568 0163-2116 |
| DOI: | 10.1007/s10620-021-07330-6 |
| Popis: | Infectious diarrhea is caused by a variety of pathogens, including viruses, bacteria, and parasitic organisms. Though the causative agent of diarrhea has historically been evaluated via stool cultures, recently, culture-independent diagnostic tests (CIDT) have been developed and utilized with increasing frequency. Current practice guidelines recommend their use as adjuncts to stool cultures for diagnosing acute and chronic diarrhea. The three principal CIDT are microscopy, enzyme-based immunoassays (EIAs), and molecular based polymerase chain reaction (PCR). This review explores the common causes of infectious diarrhea, the basics of stool culture, the diagnostic utility of these three culture-independent modalities, and the strengths and weaknesses of all currently available clinical techniques. It also outlines considerations for specific populations including returning travelers and those with inflammatory bowel disease. |
| Databáze: | OpenAIRE |
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