The formation of Cd-phytochelatin complexes in plant cell cultures
| Autor: | Meinhart H. Zenk, Ralf Kneer |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Rauvolfia
Chromatography biology Stereochemistry Chemical structure Size-exclusion chromatography Plant Science General Medicine Horticulture biology.organism_classification Biochemistry chemistry.chemical_compound Biosynthesis chemistry Rauvolfia serpentina Sephadex Chelation Phytochelatin Molecular Biology |
| Zdroj: | Phytochemistry. 44:69-74 |
| ISSN: | 0031-9422 |
| DOI: | 10.1016/s0031-9422(96)00514-6 |
| Popis: | Three distinct Cd-phytochelatin complexes were purified to apparent homogeneity by gel filtration on Sephadex G50 of crude Cd-phytochelatin preparations isolated from Cd-treated suspension cultures of Rauvolfia serpentina . These purified complexes were analysed and found to be significantly different in PC chainlength, S:Cd ratio, and sulphide content. Kinetic measurements of PC n and sulphide content in Cd-treated suspension cultures of R. serpentina and Silene cucubalus showed that there are two possible ways of decreasing the amount of sulphur (and nitrogen) needed for Cd 2+ sequestration: either by increasing the PC chainlength or by incorporating sulphide into the complexes. The latter process appears to be the more effective. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect