Patterns of mutations in nine cancer-related genes and PAF development among smoking male patients diagnosed with bladder cancer

Autor: Eman Alshehri, Amal M. Al-Dogmi, Tahani Mohamed Ibrahim Al-Hazani, Maha Abdulla Alwaili, Fatmah Ahmed Safhi, Lina Mohammed Alneghery, Areej Saud Jalal, Ibtesam Sanad Alanazi, Fatima Abdullah AlQassim, Mashael Alhumaidi Alotaibi, Wedad Saeed Al-Qahtani
Rok vydání: 2023
Předmět:
Zdroj: Tumor Biology. 45:1-14
ISSN: 1423-0380
1010-4283
DOI: 10.3233/tub-220032
Popis: BACKGROUND: Smoking is one of the most popular risk factors provoking bladder cancer (BC). This research intended to estimate cigarette smoking effect involving PAF signs between smoking patients with BC and non-smoking patients with same diagnosis to define relations with pathological characteristics and their prognosis on zero-relapse and disease-associated recovery. METHODS: Two groups of smokers (n = 54) and non-smokers (n = 62) were selected. Both cohorts of patients had BC. They were evaluated utilizing NGS on 9 cancer-related genes and confirmed through the Sanger DNA sequencing and histopathological tests based on H&E staining. The factor of smoking and impact of PAF development by ELISA assay and PAF-R manifestation in terms of immunochemical evaluation on BC areas comparing to a control group (n = 30) was examined involving healthy contributors, including the use of well-designed statistical trials. RESULTS: The multivariate evaluation showed considerable rise in mutation patterns related to smoking among BC patients (group 3), increase in PAF development (***P
Databáze: OpenAIRE
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