AMPA-elicited dark cell degeneration of cerebellar Purkinje neurons has characteristics of apoptosis

Autor: Paul Barenberg, Jean C. Strahlendorf, Kathryn K. McMahon, Barbara G. Border, Randy Miles, Howard K. Strahlendorf
Rok vydání: 1999
Předmět:
Zdroj: Neuroscience Research Communications. 25:149-161
ISSN: 1520-6769
0893-6609
DOI: 10.1002/(sici)1520-6769(199911/12)25:3<149::aid-nrc4>3.0.co;2-s
Popis: Because AMPA receptor-induced degeneration of cerebellar Purkinje cells and hippocampal pyramidal cells morphologically exhibits a type of delayed cell death often observed in numerous acute clinical conditions, understanding the mechanism of the toxicity is of major therapeutic importance. We utilized morphological and biochemical techniques in the cerebellar slice preparation to determine whether AMPA-induced dark cell degeneration of Purkinje cells represents an apoptotic-like toxicity. In addition to the previously described morphological features of DCD, additional evidence to support apoptotic mechanisms was obtained. These findings included DNA fragmentation detected by both in situ TUNEL labeling and gel electrophoresis, attenuation of AMPA-induced morphological damage using an endonuclease inhibitor, and an inhibitor of protein translation. The results of these experiments reveal that AMPA-receptor elicited toxicity of PCs is an active process that has many characteristics and features of classically defined apoptosis.
Databáze: OpenAIRE
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