Protein Storage Vacuoles Originate from Remodeled Preexisting Vacuoles in Arabidopsis thaliana
| Autor: | Rima Menassa, Chris Hawes, Lorenzo Frigerio, Maike Kittelmann, Mistianne Feeney |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Serial block-face scanning electron microscopy chemistry.chemical_classification genetic structures biology Physiology Immunoelectron microscopy Plant Science Vacuole biology.organism_classification 01 natural sciences Cell biology 03 medical and health sciences 030104 developmental biology chemistry Arabidopsis Organelle Genetics Arabidopsis thaliana Storage protein Lytic vacuole circulatory and respiratory physiology 010606 plant biology & botany |
| Zdroj: | Plant Physiology. 177:241-254 |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.18.00010 |
| Popis: | Protein storage vacuoles (PSV) are the main repository of protein in dicotyledonous seeds, but little is known about the origins of these transient organelles. PSV are hypothesized to either arise de novo or originate from the preexisting embryonic vacuole (EV) during seed maturation. Here, we tested these hypotheses by studying PSV formation in Arabidopsis (Arabidopsis thaliana) embryos at different stages of seed maturation and recapitulated this process in Arabidopsis leaves reprogrammed to an embryogenic fate by inducing expression of the LEAFY COTYLEDON2 transcription factor. Confocal and immunoelectron microscopy indicated that both storage proteins and tonoplast proteins typical of PSV were delivered to the preexisting EV in embryos or to the lytic vacuole in reprogrammed leaf cells. In addition, sectioning through embryos at several developmental stages using serial block face scanning electron microscopy revealed the 3D architecture of forming PSV. Our results indicate that the preexisting EV is reprogrammed to become a PSV in Arabidopsis. |
| Databáze: | OpenAIRE |
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