Effect of Recombinant Hirudin on Cytosolic Ca2+ Concentrations using Different Platelet Stimulators

Autor:	H.K. Breddin, O.K. Bellinger, Carl M. Kirchmaier, B. Schulz
Rok vydání:	1991
Předmět:	R hirudin chemistry.chemical_element Hematology Calcium Cytosol Thrombin Recombinant Hirudin chemistry Biochemistry Physiology (medical) medicine Under-stimulation Platelet Cytosolic calcium medicine.drug
Zdroj:	Pathophysiology of Haemostasis and Thrombosis. 21:121-126
ISSN:	1424-8840 1424-8832
Popis:	Cytosolic Ca2+ concentration, [Ca2+]i, and aggregation behavior of hirudinized platelet suspensions were examined under stimulation with thrombin, adinosine diphosphate, platelet-activating factor, collagen and the calcium ionophore, ionomycin. A rise in [Ca2+]i, a key step in platelet activation, was only inhibited by recombinant hirudin r-hirudin using the agonist thrombin. All other stimulators including ionomycin were not affected by r-hirudin. If hirudinized blood was gel-filtered before platelet stimulation, thrombin induced the same response as citrated platelet suspensions. Aggregation studies and calcium influx using various stimulators showed good correlation: only thrombin-induced aggregation was completely inhibited by r-hirudin. These results support the hypothesis that hirudin inhibits platelet function by thrombin binding and not by direct interaction with platelets.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::01936f6a551e4db4d3eb3c3738f788e3 https://doi.org/10.1159/000216272 Zobrazit plný text záznamu