| Popis: |
The aim of the study was to monitor the changes observed in the concentration of gastrin, as well as the morphological and ultrastructural properties of pyloric G and fundic ECL cells in rats with a portocaval shunt (PCS). Eight weeks after surgery, plasma and pyloric tissue gastrin concentrations were determined by radioimmunoassay. Sections from pyloric mucosa were immunostained for the identification of G cells, while oxyntic mucosa specimens were processed for Sevier-Munger detection of ECL cells. In addition, ultrastructural properties of G and ECL cells were determined by standard transmission electron microscopy. The results showed that the plasma gastrin levels were unchanged, while the pyloric gastrin concentrations were significantly increased in rats with PCS compared to the control animals (p |
