| Autor: |
Qamar Ayesha, Aleem Ahmed Khan, Subhra Lahiri, Chittoor Mohammed Habibullah |
| Rok vydání: |
1995 |
| Předmět: |
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| Zdroj: |
International Hepatology Communications. 4:183-189 |
| ISSN: |
0928-4346 |
| DOI: |
10.1016/0928-4346(95)00238-3 |
| Popis: |
The microencapsulation technique is being used successfully in various types of cells for providing a substratum for long-term survival and immunoprotection to immobilized transplanted cells. Rat hepatocytes were microencapsulated in alginic acid poly-l-lysine membrane and transplanted intraperitoneally in rabbits. The animals were followed up to study the functional capacity of transplanted hepatocytes by assessing ureagenesis and the efficacy of the capsule membrane to provide a physical barrier between the host's immune system and the encapsulated hepatocytes, assessed by no evidence of hepatocyte rejection by the host for up to 2 months. The transplanted animals were sacrificed by cervical dislocation after 15, 30, 45 and 60 days. The transplanted capsules were retrieved and the hepatocytes were studied for their capacity for ureagenesis. The capsules were found intact even after 60 days of transplantation. The retrieval was 75% with 80% of viable cells displaying normal functional capacity to produce urea. Therefore, it may be concluded that the microencapsulation technique is effective in maintaining functional capacity and providing a physical barrier between the host's immune system and immobilized cells. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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