Monoclonal antibodies to P epitope of chordin detect type II astrocytes in glial cultures

Autor:	N. V. Slepko, L. I. Kondakova, A. A. Preobrazhenskii, A. S. Khalanskii, V. U. Kalenchuk, M. V. Kozlova, N. K. Isaev
Rok vydání:	1996
Předmět:	biology medicine.drug_class General Medicine Monoclonal antibody Molecular biology General Biochemistry Genetics and Molecular Biology Epitope medicine.anatomical_structure nervous system Cell culture Polyclonal antibodies Monoclonal medicine Hepatic stellate cell biology.protein Chordin Astrocyte
Zdroj:	Bulletin of Experimental Biology and Medicine. 122:838-841
ISSN:	1573-8221 0007-4888
DOI:	10.1007/bf02445164
Popis:	The expression of chordin P-epitope in dissociated cell culture of rat brain is studied by indirect immunofluorescence. Specific fluorescence is confined to compact bipolar and stellate cells on a layer of P-negative spread cells. Granular cells of the cerebellum, spinal neurons, neurons, and Schwann cells are P-negative. Double staining with monoclonal and polyclonal antibodies to glial fibrillar acid protein (an astrocyte marker) shows that this protein is expressed by P-positive stellate cells and P-negative cells of the underlying monolayer. Thus, monoclonal antibodies to P-epitope detect type II astrocytes.
Databáze:	OpenAIRE
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