The study of a fluorescent biosensor based on polyelectrolyte microcapsules with encapsulated glucose oxidase
| Autor: | L. I. Shabarchina, L. I. Kazakova, N. P. Sirota, T. V. Sirota |
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| Rok vydání: | 2017 |
| Předmět: |
Tris
Polarography biology chemistry.chemical_element 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Electrochemistry 01 natural sciences Fluorescence Polyelectrolyte 0104 chemical sciences Ruthenium chemistry.chemical_compound chemistry biology.protein Glucose oxidase Physical and Theoretical Chemistry 0210 nano-technology Biosensor Nuclear chemistry |
| Zdroj: | Russian Journal of Physical Chemistry A. 91:1828-1832 |
| ISSN: | 1531-863X 0036-0244 |
| DOI: | 10.1134/s0036024417090151 |
| Popis: | A fluorescent biosensor is synthesized and described. The biosensor consists of polyelectrolyte microcapsules with glucose oxidase (GOx) entrapped in the cavities and an oxygen-sensitive fluorescent indicator Ru(dpp) immobilized in shells, where Ru(dpp) is tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride. The theoretical activity of the encapsulated GOx and the effect storage time and medium composition have on the stability of sensor microcapsules are determined from polarographic measurements. No change in the activity of the encapsulated enzyme and or its loss to the storage medium are detected over the test period. The dispersion medium (water or a phosphate buffer) are shown to have no effect on the activity of microcapsules with immobilized GOx. The described optical sensor could be used as an alternative to electrochemical sensors for in vitro determination of glucose in the clinically important range of concentrations (up to 10 mmol/L). |
| Databáze: | OpenAIRE |
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