Discovery of Alpha-1-Proteinase Inhibitor Binding Peptides from the Screening of a Solid Phase Combinatorial Peptide Library
| Autor: | D. H. Hammond, P. D. Bastek, George A. Baumbach, Ruben G. Carbonell, J. M. Land |
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| Rok vydání: | 2000 |
| Předmět: |
Chromatography
Aqueous solution Chemistry Process Chemistry and Technology General Chemical Engineering Alpha (ethology) Filtration and Separation General Chemistry Human serum albumin Affinity chromatography Blood plasma Protein purification medicine Blood plasma fractionation Peptide library medicine.drug |
| Zdroj: | Separation Science and Technology. 35:1681-1706 |
| ISSN: | 1520-5754 0149-6395 |
| Popis: | Alpha-1-proteinase inhibitor (A1PI, also known as alpha-1-antitrypsin), a blood plasma protein, was radiolabeled and screened against a portion of a hexameric, solid-phase combinatorial peptide library. The screening of 2% of a library containing 34 million peptides yielded 19 sequences. All 19 sequences bound A1PI when immobilized to a chromatographic support, but with varying avidities. Several of these sequences proved capable of purifyingA1PI from aqueous mixtures with human serum albumin and from Effluent II+III, a process intermediate in the Cohn plasma fractionation process. A single chromatography step was shown to provide high yields with significant purification and no measurable loss in activity. |
| Databáze: | OpenAIRE |
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