| Autor: |
Lv Yu-cai, Tang Jingting, De-ying Li, Da Chun Gong, Tian Hui, Zhao Ru-ming, Gong Mei-zhen |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
2011 International Conference on New Technology of Agricultural. |
| Popis: |
The commercial cellulose was purified by means of (NH 4 ) 2 SO 4 depositon, Sephadex G-150 chromatography and DEAE-Sephadex1–50.The specific activity of the β-glucosidase increased 93.13 fold and the coefficient of recovery was 6.08%, the molecular mass was 53kDa determined by gel imaging system after SDS-PAGE. The specific activity of the exo-β-glucanase increased 3.86 fold and the coefficient of recovery was 2.02%, the molecular mass was 33kDa determined by gel imaging system after SDS-PAGE. The specific activity of the endo- β-glucanase increased 4.07 fold and the coefficient of recovery was 2.6%, the molecular mass was 64kDa determined by gel imaging system after SDS-PAGE. The high filter activity can be obtained by the cocktail mixture in accordance with the specific proportion of the three kinds of enzyme endo-β-glucanase:β-glucosidase: exo—β-glucanase 3∶2∶15. Mg2+, Fe3+ can effectively activate the three kinds of enzyme at the meantime. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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