UV-B radiation induced effects on human T-lymphocytes

Autor:	Agostina Congiu Castellano, D. Pozzi, P. Grimaldi, Gianfranco Giubileo, S. Gaudenzi, Adriana Puiu
Rok vydání:	2007
Předmět:	medicine.diagnostic_test Chemistry Analytical chemistry Laser Radiation effect Jurkat cells Fourier transform spectroscopy law.invention Flow cytometry law Biophysics medicine Fourier transform infrared spectroscopy Spectroscopy Photoacoustic spectroscopy
Zdroj:	SPIE Proceedings.
ISSN:	0277-786X
DOI:	10.1117/12.757866
Popis:	The reactive oxygen species (ROS) produced inside cells by UV-B radiation may induce apoptosis, a process that realizes the programmed death of cells. In the present paper the UV induced damage was studied in a human T-Lymphocyte cell line (the Jurkat line) by mean of IR laser photoacoustic spectroscopy (PAS) and by Fourier Transform Infrared Spectroscopy (FTIR) combined with biological assays based on flow cytometry. The apoptosis was induced in vitro in the Jurkat samples by exposition to UV-B radiation with a dose of 310 mJ/cm2. PAS measurements were performed through a 10W c.w. CO2 laser based optical system realised at ENEA Molecular Spectroscopy Laboratory in Frascati (Italy).© (2007) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Databáze:	OpenAIRE
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