| Autor: |
Patricia Mestries, Denis Barritault, Carine Strup, P. Kern, Catherine Alexakis, Denis Mathé, Jean-Pierre Caruelle |
| Rok vydání: |
2004 |
| Předmět: |
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| Zdroj: |
Journal of Biomedical Materials Research Part A. :594-602 |
| ISSN: |
1549-3296 |
| Popis: |
Restenosis is characterized by vascular smooth muscle cell (VSMC) proliferation and accumulation of col- lagen III in a hypertrophic and disorganized extracellular matrix. Restenosis is prevented by antimitotic agents or irradiation but no significant progress has been made to control collagen expression deregulation. Previously, we have shown that a new family of biopolymers named RGTA (heparan mimetics elaborated by grafting on dextran of carboxylate, sulfate, and benzylamide units) stimulate in vivo tissue repair and reduce fibrosis in various models. Using VSMC in vitro (pig aortic VSMC irradiated with a 60 Co source and labeled with ( 3 H)Proline), we now show that gamma-irradiation reduced cell survival by 50% and colla- gen synthesis 6-fold with a major increase in the ratio of collagen III to collagen I biosynthesis taken as a fibrotic index. RGTA added to the cells enhanced their survival up to 80% and reduced collagen III/I ratio back to values found in normal vascular tissues. These results suggest that RGTA combined with gamma-radiation could be an efficient strat- egy against restenosis. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 70A: 594 - 602, 2004 |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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