| Autor: |
Nobu Kikuchi, Kyoko Matsuno, Taihei Miki |
| Rok vydání: |
1993 |
| Předmět: |
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| Zdroj: |
Analytica Chimica Acta. 283:338-343 |
| ISSN: |
0003-2670 |
| DOI: |
10.1016/0003-2670(93)85241-b |
| Popis: |
Recent interest in assaying betaine in plant tissue stimulated work on the complete separation of this substance from numerous impurities. A suitable separation of betaine was achieved by liquid chromatography on an amino-bonded silica gel. Pretreatment of samples of an aqueous extract of Jikoppi, an oriental medicine, was primarily based on a method developed by Vialle et al. in which an extract of Jikoppi was passed through a Dowex 50W-X8 (H + ) column. The betaine retained in the column was eluted with 4% ammonia solution. The solution was then purified by passing it through column containing a mixed bed of Amberlite IRC-50 [H + ] and Dowex 1-X4 (OH − ). The combined effluent and washings from the purification column were evaporated into a syrup. A 15-mg portion of glycine was added to this condensed product as an internal standard. A laboratory-packed Kaseisorb LC NH 2 super (5 μm) column satisfactorily separated betaine and glycine from impurities using acetonitrile-water (75 + 25) containing 0.1% of Brij-35 with a detection wavelength of 210 nm. Linearity of the calibration based on the peak-area ratio was demonstrated over the entire working range of 0.25–18 μg μl −1 . The relative 95% fiducial limits of betaine were determined to be 1.3–1.6% for the 0.5–12-μg range and 0.5–1.2% for the 14-18-μg range ( n =5). The recovery of betaine through the whole analytical procedure was 93% ( n =5). Betaine contents in six Jikoppi powders were also determined. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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