Characterization of N-acetyl-D-glucosamine uptake in Aphanocladium album

Autor:	Valerie Vasseur, Gilbert Bompeix, Thierry Barchietto
Rok vydání:	1994
Předmět:	Strain (chemistry) biology Immunology Mutant Aminoglycoside General Medicine Fungi imperfecti Metabolism Fungus Membrane transport biology.organism_classification Applied Microbiology and Biotechnology Microbiology Biochemistry Labelling Genetics Molecular Biology
Zdroj:	Canadian Journal of Microbiology. 40:461-466
ISSN:	1480-3275 0008-4166
DOI:	10.1139/m94-075
Popis:	A mutant of the fungus Aphanocladium album, characterized as chitinase-overproducing, does not grow on medium containing N-acetyl-D-glucosamine as sole carbon source. The lack of growth may indicate a defect either in the uptake system or in any subsequent step in the metabolism of N-acetyl-D-glucosamine. Since transport is the first step at which control of metabolism could operate, the mutant strain was studied for its ability to transport N-acetyl-D-glucosamine. The results were compared with those obtained with the wild-type strain. Labelling experiments, designed to characterize N-acetyl-D-glucosamine uptake, indicated that uptake was due to the simultaneous operation of two transport systems, one of high affinity and one of low affinity. Wild-type and mutant strains displayed different Km values for the low-affinity system. The Km value of the mutant's low-affinity system was very high compared with that of the wild-type strain.Key words: deuteromycete, filamentous fungus, Aphanocladium album, uptake, N-acetyl-D-glucosamine.
Databáze:	OpenAIRE
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