The genotypic response towards haploid induction in Turkish onion (Allium cepa L.) germplasm

Autor: Faika Yarali Karakan
Rok vydání: 2020
Předmět:
Zdroj: Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 48:1176-1184
ISSN: 1842-4309
0255-965X
DOI: 10.15835/nbha48311964
Popis: Haploid induction efficiency of unpollinated flower buds in twenty-six Turkish onion germplasm were determined on two media, Dunstan and Short medium (BDS) and Murashige and Skoog’s medium (MS). On BDS medium, 5850 flower buds were cultured and the induction rate was 2.10% and a plant survival percentage of 1.62% (2 plants). On MS medium, 5850 flower buds were cultured and 175 plantlets were induced; induction rate was 2.99% and plant survival percentage was 13.71% (24 plants). The highest number of plantlets was obtained from genotype Sanliurfa 2; 27 plantlets on BDS medium while 45 plantlets on MS medium. Flow cytometry confirmed that out of the twenty-six plants, 4 plants were haploid (15.38%), 2 mixoploid (7.69%), 18 (69.23%) diploid and 2 tetraploid (7.69%).
Databáze: OpenAIRE