Autor:	G. Candiano, N. Castagnino, Francesco Attanasio, R. Ponassi, M. Schülein, Giovanni Rialdi, Maurizio Bruschi
Rok vydání:	2001
Předmět:	Electrophoresis Crystallography Isoelectric point Chemistry Isoelectric focusing Analytical chemistry Titration Protonation Isothermal titration calorimetry Isothermal process Macromolecule
Zdroj:	Journal of Thermal Analysis and Calorimetry. 66:123-132
ISSN:	1418-2874
DOI:	10.1023/a:1012439732130
Popis:	Titration of ionizable groups of D10N variant catalytic domain has been performed by isothermal calorimetric technique and by combined isoelectric focusing-electrophoresis in the range of pH 3–9. pK0i andΔH0i related to the ionizable groups have been estimated using Lindestrom–Lang equation. Both experimental techniques are in agreement and confirm the validity of the applied theory. Slight differences in protonation enthalpies of carboxyl groups are caused by Asp10 interaction with the other part of the macromolecule.
Databáze:	OpenAIRE
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